Automatic Detections of Microscopy Tasks

The open-source computer software called the Micropilot is the newest entrant in the software programs that automates complex microscopy experiments quickly once it detects cell features. The Micropilot software quickly evaluates low-resolutions imageries taken from a microscope and, once a cell or a structured of interest had been identified, it instructs the microscope to automatically start an experiments, which can be as modest as a simple recording of detailed images of time-lapse videos, or can be as multifaceted as using lasers in probing fluorescently labeled proteins and recording its specific results.
A team at the European Molecular Biology Laboratory in Heidelberg, Germany that includes Ranier Pepperkok and Jan Ellenberg designed the Micropilot, had been using programs and applications to position numerous different experimentations probing different features of a cell structure. As a result, they have determined and consequently established the development of a structure known as endoplasmic reticulum exit sites, and uncovered the particular roles of two proteins, namely the CENP-E and the CBX1. They found out the structures by condensing genetically composed materials into firmly looped chromosomes and then forming the spindle that helps align those looped chromosomes. Finally in four nights of unattended microscopic operation, Micropilot identified 232 structured cells in two particularly specific stages of cell separation and then automatically performing itself a complex imaging experiments. Ideally, an experienced microscopic technician would have to work a full month just to simply to find those cells amongst the thousands in the sample.
Note: The experiments and computer software works are detailed in Nature Methods.


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